植物病理系

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姓名:武晓云

职称: 副教授

学科领域:植物病理学

研究方向:植物病毒学

邮箱: wxy5551@126.com

个人简历

 

学习经历:

1998.09-2002.07,东北农业大学植物保护教育专业,农学学士;

2004.09-2009.07,云南农业大学植物病理学专业,农学博士;

2007.04-2009.07,浙江大学生物技术研究所,联合培养;

2013.09-2013.12,上海外国语大学留学培训部,英语培训班学习

作经历:

2009.08-2010.06,浙江林学院农业与食品科学学院,讲师;

2010.06-2012.08,浙江农林大学农业与食品科学学院,讲师;

2012.09-2017.12,浙江农林大学农业与食品科学学院,副教授;

2015.10-2016.11,加拿大农业部伦敦研发中心,访问学者;

2018.01-2022.11,东北农业大学农学院,副教授;

2022.12-至今,必威,副教授.

教学工作

授课情况:

本科生课程:《农业微生物学》《农业微生物学实验》《分子生物学》

研究生课程:《高级植物病理学研究方法》《SCI科技论文写作》

科研工作

科研项目:

1.“大豆抗苜蓿花叶病毒种质资源筛选与鉴定”黑龙江省自然科学基金联合引导项目(LH2019C027),2019.07-2022.07.主持人.10万

2.“苜蓿花叶病毒对大豆的致病机制及大豆抗性资源挖掘研究”中央支持地方高校改革发展资金人才培养支持计划项目,2019.12-2021.12.第二.100万

3.“植物病毒抑制寄主系统性获得免疫的分子机理及应用研究”黑龙江自然科学基金重点项目(ZD2018002),2018.07-2021.07.第二.20万

4.“大豆抗苜蓿花叶病毒资源鉴定与抗性基因定位研究”东北农业大学第四批“学术骨干”项目(18XG04),2018.10-2020.10.主持人.10万

5.“烟草10个基因的克隆、载体构建与遗传转化”贵州烟草研究院项目,2016.11-2017.12.主持人.10万

6.“国家留学基金委出国留学项目”国家留学基金委([2014]3012),2015.10-2016.10.主持人

7.“利用人工ta-siRNA表达体系在水稻中进行多价抗病毒的研究”国家自然科学基金青年基金项目(31101415),2012.01-2014.12.主持人.23万

8.“基于拟南芥TAS基因的ta-siRNA表达载体的构建与抗病毒评价”浙江省自然科学基金一般项目(Y3110277),2010.01 -2012.12.主持人.8万

9.桑树雌雄植株MMLRaV侵染相关mal-miR01的功能分析,浙江省自然科学基金(Q17C160011)2016.10-2019.12.第三.8万

10.小麦叶枯病菌致病相关基因的分离与鉴定,浙江省自然科学基金(Y3110316),2012.10-2014.10.第三.8万

11.灰霉病菌啶酰菌胺抗药性突变体对甲氧基丙烯酸酯类杀菌剂表现负交互抗药性的机制,国家自然科学基金面上项目(31071711),2011-2013.第二.23万

12.浙江省蔬菜主要病害抗药性的普查、监测及治理技术开发,浙江省科技厅公益性项目,2011.01-2013.12.第二.15万

13.“番茄环斑病毒传播介体的确定及其防治策略的研究”浙江农林大学校科研发展基金,2010.01-2012.12.主持人.0.5万

14.浙江农林大学人才启动基金,2009.主持人.4万

论文著作

1.Xue Jiang, Yameng Luan, Mengzhu Chai, Yingshuai Yang, Yuting Wang, Wenjia Deng, Yonggang Li, Xiaofei Cheng*,Xiaoyun Wu*. The N-terminal α-helix of potato virus X-encoded RNA-dependent RNA polymerase is required for membrane association and multimerization. Viruses, 2022, 14: 1907.(中科院三区,5.818)

2.Xiaoyun Wu, Mengzhu Chai, Jiahui Liu, Xue Jiang, Yingshuai Yang, Yushuang Guo, Yong Li*, Xiaofei Cheng*. Turnip mosaic virus manipulatesDRM2expression to regulate host CHH and CHG methylation for robust infection. Stress Biology, 2022, 2: 29.

3.Xiaofei Cheng, Yameng Luan,Xiaoyun Wu. Plant nonhost resistance against viruses: current status and future prospects. In: Guar RK, Khurana SMP, Sharma P, Hohn T eds. Plant virus-host interaction-molecular approaches and viral evolution (second edition). Academic press. London, UK. 2021, 45-57.

4.孙婷,王馨玥,刘洪义,程晓非,武晓云*.马铃薯奥古巴花叶病毒RT-PCR检测方法的建议及应用.植物检疫,2021,35(3):47-52.

5.Xiaoyun Wu, XiaofeiCheng*. Intercellular movement of plant RNA viruses: Targeting replication complexes to the plasmodesma for both accuracy and efficiency. Traffic, 2020, 21(12): 725-736.(中科院二区,6.144)

6.Xinyang Che, Xue Jiang,Xinlei Liu, Xiaoyan Luan, Qi Liu, Xiaofei Cheng*,Xiaoyun Wu*. First report of alfalfa mosaic virus on soybean in Heilongjiang, China. Plant Disease, 2020, 104(11): 3085.(中科院二区,3.955)

7.Xiaoyun Wu, Jiahui Liu,Xiaofei Cheng*. First report of camellia japonica associated emaravirus 1associated with camellia leaf ringspot and flower color-breaking disease in China. Plant Disease, 2020, 104(12): 3271.(中科院二区,3.955)

8.Mengzhu Chai+,XiaoyunWu+, Jiahui Liu, Yue Fang, Yameng Luan, Xiaoyan Cui, Xueping Zhou, Aiming Wang, Xiaofei Cheng*. P3N-PIPO interacts with P3 via the shared N-terminal domain to recruit viral replication vesicles for cell-to-cell movement. Journal of Virology, 2020, 94(8): e01898-19

9.Ruonnan Zhang+,Xiaoyun Wu+, Xue Jiang, Xiaoxia Wu, Xiaoyan Luan, Xiaofei Cheng*. Molecular characterization of Common bean curly stunt virus: a novel recombinant geminivirus in China. Archives of Virology, 2020, 165: 257-260.(中科院二区,6.549)

10.Jiahui Liu, Lili Zhang, Feifei Xu FF, Mengzhu Chai, Xiaodong Wu, Unsong Kim, Dianqiu Lv,Xiaoyun Wu, Xiaoxia Wu, Xiaofei Cheng*. Molecular analysis of a divergent isolate of potato virus H from potato reveals novel evolutionary feature of Carlaviruses. Canadian Journal of Plant Pathology, 2020, 42: 116-124.(中科院四区,2.074)

11.Xiaoyun Wu, Adrian Valli, Juan Antonio Garcia, Xueping Zhou, Xiaofei Cheng*. The tug-of-war between plants and viruses: great progress and many remaining questions. Viruses, 2019, 11: 203(中科院三区,5.818)

12.Xiaoyun Wu, Jiahui Liu, Chai Mengzhu, Jinhui Wang, Dalong Li, Aiming Wang, Xiaofei Cheng*. The potato virus X TGBp2 protein plays dual functional roles in viral replication and movement. Journal of Virology, 2019, 93: e01635-18.(中科院二区,6.549)

13.Xiaoyun Wu, Qi Liu, Chai Mengzhu, Lili Zhang, Xiaofei Cheng*, First report of Potato aucuba mosaic virus on potato in China. Plant Disease, 2018 102(12): 2671.(中科院二区,3.955)

14.Xiaodong Wu,Xiaoyun Wu, Wenbin Li, Xiaofei Cheng*. Molecular characterization of a divergent strain of calla lily chlorotic spot virus infecting celtuce (Lactuca sativavar. augustana) in China. Archives of Virology, 2018, 163: 1375-1378.(中科院四区,2.685)

15.Xiaoyan Cui, Hoda Yaghmaiean, Guanwei Wu,Xiaoyun Wu, Bin Chen, Thorn G, Aiming Wang. The C-terminal region of theturnip mosaic virus P3 protein is essential for viral infection via targeting P3 to the viral replication complex. Virology, 2017, 510: 147-155(中科院三区,3.513)

16.蔡健宇,武晓云,郭玉双,李翔宇.烟草基因组中AGO蛋白的生物信息学分析,江西农业学报,2017,29(2):1-8.

17.詹琳琳,郭玉双,蔡健宇,武晓云,杨静. NtMAP70基因在烟草抗马铃薯Y病毒过程中的作用,福建农林大学学报(自然科学版),2016,45 (5) :556-560.

18.Linyun Chen, Xiaofei Cheng, Jianyu Cai, Linlin Zhan, Xiaoxia Wu, Qi Liu,Xiaoyun Wu*. Multiple virus resistance using artificial trans-acting siRNAs. Journal of Virological Methods, 2016, 228: 16–20.(中科院四区,2.623)

19.Xiaofei Cheng, Fangfang Li, Jianyu Cai, Wei Chen, Nan Zhao, Yuqiang Sun, Yushuang Guo, Xiulin Yang,Xiaoyun Wu*. Artificial TALE as a convenient protein platform for engineering broad-spectrum resistance to begomoviruses. Viruses, 2015, 7: 4772-4782.(中科院三区,5.818)

20.Yilun Wang, Xiaofei Cheng, Xiaoxia Wu, Aiming Wang,Xiaoyun Wu*. Characterization of complete genome and small RNA profile of pagoda yellow mosaic associated virus, a novel badnavirus in China. Virus Research, 2014, 188: 103-108.(中科院三区,6.286)

21.Wei Chen, Yongsheng Qian,Xiaoyn Wu, Yuqiang Sun, Xiaoxia Wu, Xiaofei Cheng*. Inhibiting replication of begomoviruses using artificial zinc finger nucleases that target viral-conserved nucleotide motif. Virus Genes, 2014,48: 494-501.(中科院四区,2.198)

22.Xiaofei Cheng, Nasar Virk, Wei Chen, Shuqing Ji, Shuxian Ji, Yuqiang Sun,Xiaoyun Wu*. CpG usage in RNA viruses: data and hypotheses. Plos One, 2013, 8(9): e74109.(中科院三区,3.752)

23.Xiaofei Cheng,Xiaoyun Wu, Huizhong Wang, Yuqiang Sun, Yongsheng Qian, Luo Lu. High codon adaptation in citrus tristeza virus to its citrus host. Virology Journal, 2012, 9: 113.(中科院三区,5.913)

24.Xiaoyun Wu, Haitao Ding, Liping Ke, Yueyong Xin, Xiaofei Cheng. Characterization of an acid-resistant glucose 1-dehydrogenase fromBacillus cereusvar. mycoides, Romanian Biotechnological Letters, 2012, 17(4): 7540-7548.

25.Xiaofei Cheng, Jianxiang Wu, Xinying Ma, Huafu Fu,Xiaoyun Wu*. First report of citrus tristeza virus inCitrus changshanensiscv. Huyou in Zhejiang, China. Journal of plant Pathology, 2011, 93(s4): 68.(中科院四区,1.217)

26.武晓云,刘琦,郭玉双,武小霞.2011.胡柚上柑橘衰退病毒的分子检测.东北农业大学学报,42(7):117-121.

27.Xiaoyun Wu, Jianxiang Wu, Xiaofei Cheng*. First report of natural infection of broad bean wilt virus-2 onLactuca indica. Journal of Plant Pathology, 2010, 92(s4): 119.(中科院四区,1.217)

28.Xiaoyun Wu, Xueping Zhou, Zhengyue Li, Wennan Gao, Jianxiang Wu*. Monoclonal antibodies against tobacco rattle virus and its application in the detection of TRV isolates. Indian Journal of Virology, 2009, 20(2): 64-69.

29.Xiaoyun Wu,HongruiZhang, Zhengyue Li. Occurrence and seasonal pattern of western flower thrips,Frankliniella occidentalis(Pergande) (Thysanoptera: Thripidae) in Yunnan province. Acta Phytopathologica et Entomologica Hungarica, 2009, 44(1): 135-146.

30.武晓云,程晓非,张仲凯,桂富荣,李正跃.2009.西花蓟马(Frankliniella occidentalis)rDNA ITS2和CO I基因5’末端序列的克隆与比较分析.浙江大学学报(农业与生命科学版),35(4):355-364.

科研成果

专利:

1.授权发明专利:一种苜蓿花叶病毒的侵染性克隆、重组农杆菌及构建方法(排名第2),2022-01-11,中国ZL202010461785.8.

2.授权发明专利:一种马铃薯X病毒弱毒疫苗及其制备方法与应用(排名第2),2021-12-21,中国,ZL201811376177.6.

3.授权发明专利:检测烟草脆裂病毒的单克隆抗体细胞株和检测试剂盒(排名第3),2010-09-01,ZL200810225905.

4.授权发明专利:蓟马昆虫室内饲养罐(排名第3),2007-02-07,中国,ZL200520100047.